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07.09.2011  
Cell Signaling tools
Merck Millipore provides a number of new products for target identification and pathway detection/profiling, including Antibodies, Immunoassays, Cell Based Assays, High Content Analysis as well as Flow Cytometry Assays and Systems... Browse...
 
Cell Signaling Antibodies
 
Cell Signaling Antibodies, Proteins, Kits and Assays
 
Anti-EGF (Catalogue No. 07-1432)
 
EGF staining on colon
cancer tissue via anti-EGF (07-1432) and the Merck Millipore IHC Select® HRP-DAB detection system.
Epidermal growth factor (EGF) has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to be a membrane-bound molecule that is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide.
 
IGF-1R STAR ELISA Kit (Catalogue No. 17-481)
 
IGF-1R standard curve shows that the STAR ELISA kit provides useful detection in the range of 0-2.5 ng/mL IGF-1R.
IGF-1 Receptor (IGF-1R) may induce apoptosis, presumably by reduction of free IGF. IGF-1R is not as mitogenic as other growth factor receptors, but its ability to activate the PI3 Kinase pathway is critical for cell survival. The STAR (Signal Transduction Assay Reaction) ELISA is a fast, sensitive, colorimetric, and quantitative assay for IGF-1R in cell extracts. The entire assay takes less than five hours with minimal manual intervention.
 
FlowCellect Chemokine Receptor CXCR4 Surface Kit
(Catalogue No. FCXR400423)
 
Selective and quantitative detection of CXCR4 expression on the surface of Jurkat T cells, compared to ChemiSCREEN cells expressing recombinant CXCR4 and null control cells.
FlowCellect GPCR kits are optimized for use with guava benchtop flow cytometry systems, providing a fast, easy, and accurate way of characterizing GPCR expression. This kit facilitates identification of a native CXCR4-expressing cell for biological assay development and production of clonal stable cell lines. Using the included null cells and ChemiSCREEN recombinant CXCR4 cells as negative and positive controls, changes of CXCR4 expression levels during a disease state can be monitored.
 
4G10® Platinum, Anti-Phosphotyrosine
(Catalogue No. 05-1050)
 
4G10 Platinum Anti-phosphotyrosine of EGF-treated A431 cells (right) vs. untreated (left) demonstrate activation via phosphorylation (green).
Well known for its sensitive recognition of multiple phosphotyrosines on numerous substrates, 4G10 has been validated by thousands of researchers in virtually every application and tyrosine target. To improve 4G10 further, we pooled it with the next most highly regarded phosphotyrosine antibody, clone PY20, to make 4G10 Platinum, enabling more sensitive detection on more substrates than 4G10 alone.
 
Adipogenesis Assay (Catalogue No. ECM950)
 

3T3-L1 cells induced for adipogenesis and then stained with Oil Red O demonstrated
a significant increase in adipogenesis versus uninduced control cells.

Identifying regulators of adipogenesis, the formation of adipose tissue, may enable pharmacological prevention or reversal of obesity. This assay analyzes adipogenesis in the classic 3T3-L1 model. Commonly used inducers of adipogenesis, dexamethasone, IBMX and insulin, are included in convenient, ready-to-use formulations. Staining reagents provided enable quantitative characterization of adipogenesis induction or inhibition.
 
Anti-Acetyl-Lysine (Catalogue No. 05-515)
 

Western blot analysis of sodium butyrate-treated (lane 2) and untreated (lane1) HeLa cells post acid-extraction using anti-acetyllysine (05-515).

Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to reverse gene silencing. The acetylation of non-histone proteins, such as transcription factors, as well as histone acetylation appears to be involved in this process and may result in structural transitions and specific signaling within discrete chromatin domains.
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