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28.02.2018
 
Best Protein Purification Solution!
Strep-Tactin®XT Superflow® high capacity
 
High affinity meets high capacity     Comparison of the dynamic binding capacity of Strep-Tactin® Superflow® high capacity (ST HC) with Strep-Tactin® XT Superflow® high capacity (STXT HC)  

The new Strep-Tactin®XT Superflow® high capacity is used for purification of recombinant Strep-tag®II and Twin-Strep-tag® fusion proteins under gravity flow and low pressure or FPLC.

Strep-Tactin®XT Superflow® high capacity perfectly combines the high affinity (pM range) of the 3rd generation Strep-Tactin®XT resin with the 2 to 3 times higher protein binding capacity of Strep-Tactin® high capacity and thus provides significant higher yields of highly pure Strep-tag® protein than standard Strep-Tactin®XT Superflow®. Read more
 
 
 
High yields even for low-abundant proteins
Four different proteins fused to either Twin-Strep-tag® or Strep-tag®II were purified using Strep-Tactin® Superflow® high capacity (ST HC) and Strep-Tactin®XT Superflow® high capacity (STXT HC). Each lysate was applied as concentrated extract (3 mg/ml) and as diluted cell extract (0.4 mg/ml). Strep-Tactin®XT Superflow® high capacity has the highest binding capacity in both experiments. Remarkably, lower protein concentrations in the feedstock have no significant influence on the binding capacity of Strep-Tactin®XT Superflow® high capacity, independent of the used tag.
     
A simple one-step purification results in high purity
Strep-Tactin®XT Superflow® high capacity is employed in an easy and straight forward purification cycle, which leads to highly pure proteins. As all Strep-Tactin® resins, Strep-Tactin®XT Superflow® high capacity can be regenerated and re-used at least 3-5 times.
 
  Purification of recombinant Strep-tag®II or Twin-Strep-tag® proteins via Strep-Tactin® XT Superflow® high capacity under physiological buffer conditions  
 
   
  Product
Qty
Cat.No.
CHF
 
  Strep-Tactin®XT Superflow® high capacity
50 % suspension
4ml
2-4030-002  
20ml
2-4030-010  
50ml
2-4030-025  
  Strep-Tactin®XT Superflow® high capacity gravity flow columns
5x 0,2ml
2-4031-005  
1ml
2-4032-001  
5ml
2-4033-001  
10ml
2-4034-001  
  Strep-Tactin®XT Superflow® high capacity
cartridges (for Äkta/peristaltic pumps)
1ml
2-4025-001  
5ml
2-4026-001  
Key benefits
  - Highest affinity available among affinity purification systems
- Higher protein yields compared to other Strep-Tactin® resins
- Elution of concentrated protein due to small elution volumes
- Cost efficent: Less costs of resin per mg purified protein
 
 
 

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