If you have any problems in viewing this Newsletter, please click here
Addressing Bias in Small RNA Library Preparation for Sequencing
In an attempt to mitigate bias in Small RNA Library Preparation, the new Bioo Scientific NEXTflex V3 protocol utilizes a pool of adapters with random nucleotides at the ligation boundary. This allows for robust detection of several miRNAs that evade capture by the Illumina-based methods. Results of the NEXTflex protocol do correlate best with RT-qPCR. Read the study
  • Completely gel-free protocol with normal input amounts
  • Low input (5 ng) gel-based protocol included
  • Randomized adapters reduce ligation bias, resulting in more accurate data
  • AIR™ Ligase offers greater sequencing depth
  • Use more of your RNA sample - 10.5 µL input volume
  • Barcoded primers are included in the kit allowing multiplexing of up to 48 samples
  • Greater discovery/detection rates reduces costs
  • Read White Paper (general data)
  • Read White Paper (low input amount)
Product Cat# Qty
NEXTflex™ Small RNA-Seq Kit v3 (Illumina-compatible) 5132-05 8 barcodes
5132-06 48 barcodes
For ultimate RNA purification: unbiased recovery of small RNAs (17-200nt) incl. mi RNA!
Library Preparation and Target Capture
Bioo Scientific provides a complete Illumina-compatible library prep solution designed for DNA library preparation, multiplexing, and barcode blocking, upstream of Agilent SureSelectXT or SureSelectXT2 target enrichment systems: NEXTflex™ Pre- and Post- Capture Combo Kit. This kit contains everything needed for library prep, hybridization, and post capture amplification with Agilent’s SureSelectXT or SureSelectXT2 target capture baits. Read White Paper
  • NEXTflex Rapid DNA-Seq protocol reduces library prep time
  • Index-specific blocking oligos
  • Higher percentage of on-target reads
  • Options available for multiplex and single-plex captures
  • Flexible multiplexing options with up to 96 barcodes for multiplexing
Product Cat#   Qty
NEXTflex™ Pre- and Post- Capture Combo Kit Agilent SureSelect XT Compatible 5144-61 (A), 5144-62 (B), 5144-63 (C), 5144-64 (D), 5144-65 (E), 5144-66 (F) Set A, B, C, D, E or F 16 barcodes
5144-67 (G) Set G 96 barcodes
NEXTflex™ Pre- and Post- Capture Combo Kit Agilent SureSelect XT2 Compatible, 5144-51 (A), 5144-52 (B), 5144-53 (C), 5144-54 (D), 5144-55 (E), 5144-56 (F) Set A, B, C, D, E or F 16 barcodes
5144-57 (G) Set G 96 barcodes

Lucerna-Chem respects the web and the privacy of those who use it. We do not rent or sell our mailing list to anyone.
This e-Mail is Virus checked!

If you'd rather not receive Emails about product updates or Newsletters, click HERE.