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27.01.2016
Addressing Bias in Small RNA Library Preparation for Sequencing
 
In an attempt to mitigate bias in Small RNA Library Preparation, the new Bioo Scientific NEXTflex V3 protocol utilizes a pool of adapters with random nucleotides at the ligation boundary. This allows for robust detection of several miRNAs that evade capture by the Illumina-based methods. Results of the NEXTflex protocol do correlate best with RT-qPCR. Read the study
 
   
  • Completely gel-free protocol with normal input amounts
  • Low input (5 ng) gel-based protocol included
  • Randomized adapters reduce ligation bias, resulting in more accurate data
  • AIR™ Ligase offers greater sequencing depth
  • Use more of your RNA sample - 10.5 µL input volume
  • Barcoded primers are included in the kit allowing multiplexing of up to 48 samples
  • Greater discovery/detection rates reduces costs
  • Read White Paper (general data)
  • Read White Paper (low input amount)
 
Product Cat# Qty
CHF
NEXTflex™ Small RNA-Seq Kit v3 (Illumina-compatible) 5132-05 8 barcodes
5132-06 48 barcodes
 
 
For ultimate RNA purification: unbiased recovery of small RNAs (17-200nt) incl. mi RNA!
Library Preparation and Target Capture
Bioo Scientific provides a complete Illumina-compatible library prep solution designed for DNA library preparation, multiplexing, and barcode blocking, upstream of Agilent SureSelectXT or SureSelectXT2 target enrichment systems: NEXTflex™ Pre- and Post- Capture Combo Kit. This kit contains everything needed for library prep, hybridization, and post capture amplification with Agilent’s SureSelectXT or SureSelectXT2 target capture baits. Read White Paper
 
  • NEXTflex Rapid DNA-Seq protocol reduces library prep time
  • Index-specific blocking oligos
  • Higher percentage of on-target reads
  • Options available for multiplex and single-plex captures
  • Flexible multiplexing options with up to 96 barcodes for multiplexing
Product Cat#   Qty
CHF
NEXTflex™ Pre- and Post- Capture Combo Kit Agilent SureSelect XT Compatible 5144-61 (A), 5144-62 (B), 5144-63 (C), 5144-64 (D), 5144-65 (E), 5144-66 (F) Set A, B, C, D, E or F 16 barcodes
5144-67 (G) Set G 96 barcodes
NEXTflex™ Pre- and Post- Capture Combo Kit Agilent SureSelect XT2 Compatible, 5144-51 (A), 5144-52 (B), 5144-53 (C), 5144-54 (D), 5144-55 (E), 5144-56 (F) Set A, B, C, D, E or F 16 barcodes
5144-57 (G) Set G 96 barcodes
 

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