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Common problems associated with secondary antibodies in immunoassays, such as non-specific binding, are a common occurrence – there are several reasons for this:

  • Cross-reactivity of labeled secondary antibodies with endogenous immunglobulins on the tissues or cells.
  • Reactivity of labeled secondary antibodies with immunoglobulins in the diluent.
  • Cross-species reactivity with other secondary labeled antibodies.
>>> All these issues can be easily overcome by directly labeling your primary antibody, thus eliminating the need for secondaries!
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