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06.03.2013  
 
 
     
 
     
 
     
 
Antibody Arrays - More Data, Less Sample

Many biological processes such as apoptosis, inflammation, angiogenesis, immune response and migration often accompany changes of cytokine expression levels. Because of the extensive cross-talk between cytokines, a complete analysis of biological responses and functions must be obtained through multiplex assays. Antibody arrays allow a much broader view of protein activity than can be obtained with single-target ELISAs and Western blots. Moreover, antibody array screening improves the chances for discovering key factors, disease mechanisms or biomarkers related to cytokine signaling.

 
C-Series Antibody Arrays  
 

Antibodies are spotted on nitrocellulose membrane solid supports and are handled in a very similar manner to Western blots.  Chemiluminescent signal detection.

Sandwich ELISA principle, utilizing a matched pair of antibodies, an immobilized capture antibody and a corresponding biotinylated detection antibody.

   
G-Series Antibody Arrays  
 

The antibodies are spotted on glass slide solid supports and require a laser scanner for data collection. Fluorescent signal detection.

Sandwich ELISA principle, utilizing a matched pair of antibodies, an immobilized capture antibody and a corresponding biotinylated detection antibody.

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L-Series Antibody Arrays  
 

For direct signal detection, the antigen is tagged with biotin prior to incubation with the capture antibody, what eliminates any possibility of antibody interactions. This makes this array platform ideal for high-content screening of protein expression.  Capture antibodies for L-Series may be spotted on either glass slide or membrane.

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Quantibody Arrays  
 

Quantitative multiplex ELISA arrays featuring fluorescent detection. The antibodies are spotted on glass slide solid supports. Cytokine standards are provided with the array for calculation of target protein concentrations.

Sandwich immunoassay principle.

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E-Series Antibody Arrays  
 

E-Series arrays are the very first antibody arrays on the market to utilize the competitive immunoassay method for signal detection.

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