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22.02.2012  
One-STrEP-tag System

The One-STrEP™ system (one-step purification with One-STrEP-tag on Strep-Tactin® Superflow®) is recommended for getting started. It needs one tag and one purification step only and is validated for eukaryotes and prokaryotes. Due to its excellent performance, this method yields a favorable signal-to-noise ratio in most cases. Mild elution and short washing, allow the isolation of even weekly interacting preys. More...

  • Protein purification to over 99% purity with one tag and one purification step only
  • Does not hamper protein folding or secretion nor does it interfere with protein function
  • Very low tendency of Strep-Tactin® to bind other proteins non-specifically
  • Well-suited for weak protein:protein interactions
 
   
 
Catalog# Product Name Quantity Price CHF
2-1121-001 One-STrEP Set, mammalian 1 Set pricing
2-1121-002 One-STrEP Set, E. coli 1 Set pricing
Note: All kit components are also avaiable separately
 
One-TAP System

In case the One-STrEP system provides suboptimal data the "One-TAP" system (One-tag Tandem Affinity Purification with One-STrEP-tag on Strep-Tactin® and StrepMAB-Classic) extends the options of the One-STrEP system since it adds a second independent purification step yet with the same tag. Two different purification steps may better discriminate specific from non-specific binding but bear the risk of loosing weakly interacting partners. More...

  • Only one tag minimizes influence on bait protein
  • Two independent purification steps increase signal-to-noise ratio
  • Discrimination of non-specific and weak binding partners possible (by comparing to One-STrEP method)
  • Well-suited for strong protein:protein interactions
 
   
 
Catalog# Product Name Quantity Price CHF
2-1122-001 One-TAP Set, mammalian 1 Set pricing
2-1122-002 One-TAP Set, E. coli 1 Set pricing
Note: All kit components are also avaiable separately
Two-TAP System

As the highly charged metal ion binding FLAG®-tag bears the risk of non-specific interactions, this system is recommended only if the performance of One-TAP for a given protein complex is insufficient. In the first step, One-STrEP/FLAG® double-tagged proteins are eluted from Strep-Tactin® Superflow® under native conditions with biotin, in the second step elution from anti-FLAG M2 agarose is performed with the FLAG octapeptide. More...

  • Two different tags and two independent purification steps
  • Improved purification procedure for FLAG tag users
 
   
 
Catalog# Product Name Quantity Price CHF
2-1123-001 Two-TAP Set, mammalian 1 Set pricing
2-1123-002 Two-TAP Set, E. coli 1 Set pricing
Note: All kit components are also avaiable separately
SPINE System

In addition to these non-covalent capture methods of potential preys, SPINE (Strep-Protein Interaction Experiment with Strep-tag®II) adds the possibility to covalently link the preys to its bait by formaldehyde cross-linking. This linkage is achieved in the living organism enabling a time resolved snapshot of interacting proteins. SPINE is currently validated in prokaryotes only but its adaptation to mammalian systems is under way. More...

  • Only one tag (Strep-tag® II), fast and easy one-step purification
  • Preys are covalently linked to the bait and weakly associated preys cannot escape detection
  • High-stringency wash procedure reduces background
  • Time-resolved analysis of binding processes possible
 
   
 
Catalog# Product Name Quantity Price CHF
2-1124-001 SPINE Set, E. coli 1 Set pricing
Note: All kit components are also avaiable separately
 
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