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19.10.2011  
 
Quantibody® Th17 Arrays
(human & mouse)
Th17 cells are a unique set of T helper cells characterized by the production of IL-17 (also known as IL-17A), IL-17F, and many other inflammatory cytokines. Th17 cells are pro-inflammatory and are thought to be involved in the immune response to fungal and certain bacterial infections, as well as being associated with inflammation and airway hyper-responsiveness in asthma and several autoimmune diseases, including inflammatory bowel disease, rheumatoid arthritis, multiple sclerosis, and asthma. More...
How Quantibody works
Quantibody array is an array-based multiplex sandwich ELISA system for simultaneous quantitative measurement of the concentration of multiple cytokines in serum, plasma, CSF, cell lysate, conditioned media and other body fluid. It combines the advantages of the high detection specificity/ sensitivity of ELISA and the high throughput of the arrays. More...

 
Products
   
  Quantibody® Human Th17 Array kits detect 20 Human Cytokines expressed by Th1, Th2 and/or Th17 cells:
GM-CSF, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12 (p70), IL-13, IL-17 (IL-17A), IL-17F, IL-21, IL-22, IL-23, IL-28, IFNγ, MIP-3α, TGFβ1, TNFα and TNFβ
   
  QAH-TH17-1-1 (1 slide to test 8-10 samples)
QAH-TH17-1-2 (2 slides to test 16-24 samples)
QAH-TH17-1-4 (4 slides to test 48-56 samples)
   
  Pricing
   
 
   
  Quantibody® Mouse Th17 Array kits detect 20 Mouse Cytokines expressed by Th1, Th2 and/or Th17 cells:
IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12 (p70), IL-13, IL-17, IL-17F, IL-21, IL-22, IL-23, IL-28, IFNγ, MIP-3α, TGFβ1, TNFα
   
  QAM-TH17-1-1 (1 slide to test 8-10 samples)
QAM-TH17-1-2 (2 slides to test 16-24 samples)
QAM-TH17-1-4 (4 slides to test 48-56 samples)
   
  Pricing
   
 
T regulatory cells  
 
  FOXP3

FOXP3 is a 50-55 kD transcription factor and is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers. Transduced expression of FOXP3 in CD4+/CD25- cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype.

The 150D antibody recognizes FOXP3 epitope encoded by exon 2 in humans, mouse and rat T regs. Clone 206D and 259D recognize a human FOXP3 epitope in the region of amino acids 105-235. Poly6238 recognizes both human and mouse FOXP3 and was raised against the N-terminal portion of FOXP3.

 
Treg Cell Markers  

The need for identification and sorting of live Treg cells has led to characterization of a number of cell surface Treg markers:

  • CD127 (IL-7Rα) is a marker that is down-regulated on Tregs. In conjunction with CD4 and CD25 staining, lowly expressing CD127 cells can be clearly delineated as Tregs (data shown right).
  • Other functional Treg markers, CD39 and CD73, which are cell surface ecto-nucleotidase enzymes, have also been characterized.
  • Activated functional Tregs have also been demonstrated to upregulate LAP (TGF-β1) and GARP, while down-regulating expression of CD45RA.
 
 
**Change Notification**
 
Introducing new 5ul Test Sizes in 2012
Starting January 2, 2012, BioLegend will be changing the volume per test from 20 µl to 5 µl on our test size products for the following formats: FITC, PE, PE/Cy5, PE/Cy7, APC, and APC/Cy7. In accordance with this change, bottling vials will be changed from 5 mL amber glass bottles to 0.5 mL opaque polypropylene copolymer vials. More...
 
 
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