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10.08.2011  
Z-Competent™ E. coli strains
The Z-Competent™ E. coli strains are premade, chemically competent cells for simple and highly efficient DNA transformation (>10E8 transformants / µg pUC19) and can be used for cloning, sub-cloning, PCR fragment cloning, library construction, etc. Zymo Research's innovative Mix & Go! transformation procedure streamlines the process, eliminating long outgrowth times and the need for electroporation and heat shock. Using premade Z-Competent™ E. coli cells, you can transform cells in less than 20 seconds: simply mix DNA with cells and then spread onto solid medium −just Mix & Go!
  • Mix & Go! transformation procedure (10E8 - 10E9 transformants/μg of plasmid DNA)
  • No Heat Shock, no Lengthy Incubations, no Outgrowth
  • Simple procedure: add DNA and then spread. DNA transformation in as little as 20 seconds!
 
Cat# Product Qty Price CHF 10% Discount
T3015 Z-Competent™ E.coli Strain C600
for general cloning, plasmid isolation
10x 100uL 198.00 178.20
T3007 Z-Competent™ E.coli Strain DH5a
for general cloning, blue-white selection, plasmid isolation
10x 10 uL 198.00 178.20
T3009 96x 50uL 745.00 670.50
T3011 Z-Competent™ E. coli - Strain HB101
for general cloning, plasmid isolation
10x 100uL 198.00 178.20
T3013 96x 50uL 745.00 670.50
T3003 Z-Competent™ E. coli - Strain JM109
for general cloning, blue-white selection, plasmid isolation
10x 100uL 198.00 178.20
T3005 96x 50uL 745.00 670.50
T3017 Z-Competent™ E. coli - Strain TG1
for general cloning, blue-white selection, plasmid isolation
10x 100uL 198.00 178.20
Offer expires 31. October 2011. Discounts are not summable.
Please fill inquiry form or send email with
"SM100811"
 
 
Z-Competent™ E. coli Transformation Kit & Buffer Set

Zymo Research also provides reagents that enable researchers to make their own homemade Z-Competent™ E. coli strains using the Z-Competent E. coli Transformation Kit™ or the Z-Competent™ E. coli Transformation Buffer Set. Simply grow the E. coli strain of your choice in ZymoBroth™ medium (or SOB), wash, and then resuspend the cells in the provided uniquely formulated buffers. The cells are now ready for transformation!  View Protocol

  • Make your own highly efficient chemically competent cells: 10E8-10E9 transformants/μg of plasmid DNA for most common lab strains
  • No heat shock or related procedures: simply add DNA and spread onto a plate - Mix & Go!

ZymoBroth™ medium dramatically increases the transformation efficiencies of a broad range of E. coli strains. The figure above shows the relative transformation efficiencies for strains generated using SOB or ZymoBroth™. Generally, ZymoBroth™ enhances transformation efficiencies better for difficult-to-transform strains.



 
Cat# Product Qty Price CHF 10% Discount
T3001

Z-Competent E.coli Transformation Kit
- E. coli growth medium ZymoBroth™ is supplied
- for making up to 20 ml Z-Competent™ E. coli

20 Preps 173.00 155.70
T3002 Z-Competent E.coli Transformation Buffer Set
- E. coli growth medium ZymoBroth™ is NOT supplied
- for making up to 60 ml Z-Competent™ E. coli
60 Preps 195.00 175.50
M3015-100 ZymoBroth 100 mL 60.00 54.00
M3015-500 500 mL 165.00 148.50
Offer expires 31. October 2011. Discounts are not summable.
Please fill inquiry form or send email with
"SM100811"
email: lucerna-chem@lucerna-chem.ch
web: http://www.lucerna-chem.ch

 

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