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02.02.2011  
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  • Quick (15-20 minute) recovery of ultra-pure DNA from TAE or TBE-buffered agarose gels
  • DNA is eluted at high concentrations into minimal volumes (≥ 6µl) with no wash residue carryover
  • Eluted DNA is well suited for sequencing, ligation, PCR, labeling, restriction endonuclease digestions...
  • Omits the use of glass beads, organic denaturants, and proteinases
 
Features
  • Zymoclean™ Gel DNA recovery kits:
    DNA Size Limits: 50 bp to 23 kb
    DNA Binding Capacity: Up to 5μg total DNA per column or well
  • Zymoclean™ Large Fragment DNA recovery kit:
    DNA Size Limits: 50 bp to ~200 kb
    DNA Binding Capacity: Up to 10μg total DNA per column
Easy Procedure
  1. Add 3 volumes of ADB Buffer to each volume of gel
  2. Incubate at 37-55ºC for 5-10 minutes
  3. Transfer the mixture to the supplied column or filtration plate and spin 5-10 seconds into a collection tube/plate
  4. Add 200 μl of Wash Buffer to the column and spin for 30 seconds. Repeat wash.
  5. Add 6-10μl of water directly to the column matrix and elute ultra-pure DNA into a new collection tube/plate
 
Comparative Overview: Benchmarking
  Zymo Research Qiagen Invitrogen
Product Zymoclean Gel DNA Recovery Kit MinElute Gel Extraction Kit Purelink™
Method Spin Column Spin Column Spin Column
Minimal Elution Volume  6 µl  10 µl  30 µl
DNA Binding Capacity  5 µg  5µg  15 µg
% Recovery <10kb: 70-90%
>10kb: 50-70%
80% 80%
Size Range 50bp-23kb 70bp-4kb 40bp-10kb
Processing Time 10 min 18-20 min 30 min
CHF/prep 2.09 CHF 3.57 CHF 2.50 CHF
 
Prices
Cat# Product Quantity Price CHF
D4001S Zymoclean Gel DNA Recovery Sample Kit, Uncapped 10 Preps free
D4001 Zymoclean Gel DNA Recovery Kit, Uncapped 50 Preps 120.00
D4002 200 Preps 418.00
D4007 Zymoclean Gel DNA Recovery Kit, Capped 50 Preps 120.00
D4008 200 Preps 436.00
D4021 ZR-96 Zymoclean Gel DNA Recovery Kit 2x 96 Preps 330.00
D4022 4x 96 Preps 630.00
D4045 Zymoclean Large Fragment DNA Recovery Kit 25 Preps 110.00
D4046 100 Preps 382.00

 

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